Monday, August 23

Full explanation about anaerobic respiration and aerobic respiration,

Anaerobic Respiration

Anaerobic respiration is a way for an organism to produce usable energy, in the form of adenosine triphosphate, or ATP, without the involvement of oxygen; it is respiration without oxygen. This process is mainly used by prokaryotic organisms (bacteria) that live in environments devoid of oxygen. Although oxygen is not used, the process is still called respiration because the basic three steps of respiration are all used, namely glycolysis, the citric acid cycle, and the respiratory chain, or electron transport chain. It is the use of the third and final step that defines the process as respiration. In order for the electron transport chain to function, a final electron acceptor must be present to take the electron away from the system after it is used. In aerobic organisms, this final electron acceptor is oxygen. Oxygen is a highly electronegative atom and therefore is an excellent candidate for the job. In anaerobes, the chain still functions, but oxygen is not used as the final electron acceptor. Other less electronegative substances such as sulfate (SO4), nitrate (NO3), and sulfur (S) are used. Oftentimes, anaerobic organisms are obligate anaerobes, meaning they can only respire using anaerobic compounds and can actually die in the presence of oxygen.

Anaerobic respiration is not the same as fermentation, which does not use either the citric acid cycle or the respiratory chain (electron transport chain) and therefore, cannot be classified as respiration.

Abstract Oxyanions of arsenic and selenium can be used in microbial anaerobic respiration as terminal electron acceptors. The detection of arsenate and selenate respiring bacteria in numerous pristine and contaminated environments and their rapid appearance in enrichment culture suggest that they are widespread and metabolically active in nature. Although the bacterial species that have been isolated and characterized are still few in number, they are scattered throughout the bacterial domain and include Gram-positive bacteria, beta, gamma and epsilon Proteobacteria and the sole member of a deeply branching lineage of the bacteria, Chrysiogenes arsenatus. The oxidation of a number of organic substrates (i.e. acetate, lactate, pyruvate, glycerol, ethanol) or hydrogen can be coupled to the reduction of arsenate and selenate, but the actual donor used varies from species to species. Both periplasmic and membrane-associated arsenate and selenate reductases have been characterized. Although the number of subunits and molecular masses differs, they all contain molybdenum. The extent of the environmental impact on the transformation and mobilization of arsenic and selenium by microbial dissimilatory processes is only now being fully appreciated.

Aerobic Respiration

Aerobic respiration requires oxygen in order to generate energy (ATP). Although carbohydrates, fats, and proteins can all be processed and consumed as reactant, it is the preferred method of pyruvate breakdown from glycolysis and requires that pyruvate enter the mitochondrion in order to be fully oxidized by the Krebs cycle. The product of this process is energy in the form of ATP (Adenosine Triphosphate), by substrate-level phosphorylation, NADH and FADH2.

Simplified reaction: C6H12O6 (aq) + 6 O2 (g) → 6 CO2 (g) + 6 H2O (l)
ΔG = -2880 kJ per mole of C6H12O6

The negative ΔG indicates that the products of the chemical process store less energy than the reactants and the reaction can happen spontaneously; In other words, without an input of energy.

The reducing potential of NADH and FADH2 is converted to more ATP through an electron transport chain with oxygen as the "terminal electron acceptor". Most of the ATP produced by aerobic cellular respiration is made by oxidative phosphorylation. This works by the energy released in the consumption of pyruvate being used to create a chemiosmotic potential by pumping protons across a membrane. This potential is then used to drive ATP synthase and produce ATP from ADP. Biology textbooks often state that 38 ATP molecules can be made per oxidised glucose molecule during cellular respiration (2 from glycolysis, 2 from the Krebs cycle, and about 34 from the electron transport system). However, this maximum yield is never quite reached due to losses (leaky membranes) as well as the cost of moving pyruvate and ADP into the mitochondrial matrix and current estimates range around 29 to 30 ATP per glucose.

Aerobic metabolism is 19 times more efficient than anaerobic metabolism (which yields 2 mol ATP per 1 mol glucose). They share the initial pathway of glycolysis but aerobic metabolism continues with the Krebs cycle and oxidative phosphorylation. The post glycolytic reactions take place in the mitochondria in eukaryotic cells, and in the cytoplasm in prokaryotic cells.

Any questions do post down the comments below ty.Wilber

14 comments:

  1. the information above is good, but is very profound. I dont really understand some of it... (no offence..)

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  2. i need to know: Why a final electron acceptor must be present?

    opinion: actually, i agreed wth shiying, the information are very good but i cant understand a single thing. Maybe you all could explain it with a simpler English ?

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  3. I need to understand: How exactly do pyruvate enter the mitochondrion ?

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  4. Pyruvate is glucose that is split into half(which make 2 pyruvate) by glycolysis...

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  5. If a final electron acceptor isn't present, the cytochromes present would not be able to reduce the NADH into NAD+ again. This is because the cytochrome on the inner membrane of the mitochondria is already oxidised to the max. To continue the glycolysis process, it needs NAD+. If this isn't there, then pyruvate can be used, forming either ethanol or lactic acid in a process called ethanol or lactic acid fermentation

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  6. F.Y.I Citric acid cycle = kreb cycle

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  7. REP kokxuan:

    ans > hmm. its by .. active trasport.

    futherinfo > Pyruvate moves easily through the
    permeable outer membrane [by active trasport] but must be transported through the inner mitochondrial membrane by a protein carrier molecule.

    diagram > I managed to upload a sketch of pyruvate entering the mitochondria. http://img714.imageshack.us/img714/261/capturebsf.jpg

    hope it answers your question,
    glenn

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  8. REP 2E1scienceTLLM:

    I hope the SCIENCEINQUEST people answered your question. Thank you. But

    addingON > The final electron acceptor is oxygen. And we are talking about Anaerobic Respiration so oxygen is not required. Besides, anaerobes, can only respire using anaerobic compounds and can actually die in the presence of oxygen.

    thnks4the question

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  9. TOibrahim:

    futher info > Yea. The citric acid cycle has ALOT of names like
    - The tricarboxylic acid cycle [TCA cycle]
    - The Krebs cycle [As you mentioned]
    - The Szent-Györgyi-Krebs cycle

    This cycle is actually a series of enzyme-catalysed chemical reactions, which is important until like madd in all living cells that use oxygen as part of cellular respiration.

    thnks4 the help

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  10. Pyruvate enters the mitochondria by pyruvate dehydrogenase complex. Pyruvate in the substrate while pyruvate dehydrogenase. It first forms an enzyme-substrate complex, goes in the mitochondria and undergoes pyruvate decarboxylation to give your acetyl CoA for the use in the Krebs cycle.

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  11. Glucose broken down by the process called glycosis makes into 2 pyruvate and then enters through the mitochondrian through active transport answer to kokxuan

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  12. This comment has been removed by the author.

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  13. http://upload.wikimedia.org/wikipedia/commons/thumb/0/0b/Citric_acid_cycle_with_aconitate_2.svg/754px-Citric_acid_cycle_with_aconitate_2.svg.png

    (krebs cycle)

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  14. steps of krebs cycle. 1:condensation of Acetyl CoA with Oxaloacetate which gives you Citrate. 2: Dehydration of Citrate to cis-Aconitate. 3:Hydration of cis-Aconitate to Isocitrate. 4: Oxidative decarboxylation of Isocitrate to alpha-Ketoglutarate. 5: Oxidative decarboxylation of alpha-Ketoglutarate to Succinyl CoA. 6: Substrate-level phosphorylation of Succinyl CoA to Succinate. 7: Dehydrogenation of Succinate to Fumarate. 8: Hydration of Fumarate to Malate. 9: Dehydrogenation of Malate which gives you back Oxaloacatate to condense with Acetyl-CoA and it goes on. Usually the krebs cycle happens twice as Glycolosis break down glucose which gives you to 2 pyruvate which Oxidative decarboxylate into 2 Acetyl-CoA so krebs cycle can happen if there is glucose..::..

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